MTB Detection Kit

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MTB Detection Kit

Tuberculosis remains a major global health problem and ranks as the second leading cause of death from an infectious disease worldwide, after HIV. It is the first infectious disease declared by the World Health Organization (WHO) as a global health emergency.The diagnosis of M. tuberculosis complex is required for the definitive diagnosis of tuberculosis and optimal control.

The conventional identification of MTB isolates by culture and phenotypic characterization is widely used but it takes 4 to 6 weeks or longer as for slow growing species. Molecular genetic approaches for M. tuberculosis identification directly from clinical samples or on cultured isolates are preferred because of their high sensitivity and specificity, as well as rapid processing time.

These methods can potentially lessen the diagnostic time from weeks to days. Using the multicopy insertion sequence IS6110 for MTB detection enables highest sensitivity compared with conventional single copy genes detection methods. IS6110 sequence is very specific for Mycobacterium tuberculosis complex and thus differentiates them from other mycobacteria.

Mylab Discovery Solutions provides real time PCR based rapid detection of target sequence of M. tuberculosis complex and internal control in a single tube. The real time assay uses the Taqman fluorogenic probe based chemistry that uses the 5´ nuclease activity of Taq DNA polymerase and enables the detection of a specific PCR product as it accumulates during PCR cycles.

Kit Features

  • Direct to customer” clinical lab tests based on real time PCR
  • High sensitive and specific for detection of mycobacterium tuberculosis complex (MTC)
  • Includes all the reagents required to perform sample to Result analysis
  • Inclusion of positive and negative controls for quality check
  • Inclusion of internal control gene to assess the PCR inhibition
  • Cost effective and rapid test
  • Low contamination risk – no post-PCR product handling
  • High-throughput – can screen up to 46 samples in a single 96-well run
  • Fast – results in under 80 minutes after PCR start
  • Simple reaction setup and data interpretation

Kit Contents

  • Reagents required for PCR amplification/detection
  • Validated reaction controls
  • Plastic wares for detection kit.

Order Information

# Product Cat. No. Reaction size/kit
1 Pathodetect-MTB Detection Kit PMTBS25 25
2 Pathodetect-MTB Detection Kit PMTBS50 50
3 Pathodetect-MTB Detection Kit PMTBS100 100