PML RARA bcr1 and bcr3 Screening Kit

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PML RARA bcr1 and bcr3

Historically, Acute promyelocytic leukemia (APL or AML-M3) is one of the most lethal forms of acute myeloid leukemia with distinct clinical and histopathologic features.

Acute promyelocytic leukemia is characterized by a unique chromosomal translocation involving the retinoic acid receptor-alpha gene on chromosome 17 (RARA). In greater than 99% of cases of APL, retinoic acid receptor-alpha (RARA) gene on chromosome 17 is involved in a reciprocal translocation with the promyelocytic leukemia gene (PML) on chromosome 15, a translocation denoted as t(15;17)(q22;q12)

Depending on the location of breakpoints within the PML site, intron 6, exon 6 and intron 3, the respective PML-RARa transcript subtypes referred to as long (L or bcr1), variant (V or bcr2) and short (S or bcr3), may be formed representing 55%, 5% and 40% of the cases respectively. The presence of PML/RARA t(15;17) translocation is necessary for response to all-trans-retinoic acid and arsenic trioxide. Thus, the PML/RARA t(15;17) assay is useful for diagnosis and predicting treatment response. It is also helpful for monitoring therapeutic response and MRD and for detecting early relapse.

Kit Features

  • Easy workflow with ready-to-use solutions for “Direct to customer” clinical lab tests
  • Detection of the PML/RARA t(15;17) (q21;q22) translocation
  • a tool to detect for Acute promyelocytic leukemia (APL or AML-M3)
  • Real-time quantitative gene analysis of control gene and fusion gene transcript bcr1 (PMLex6/RAR ex3L-form (=BCR1) ) and fusion gene transcript bcr3 (PMLex6/RAR ex3S-form (=BCR3))
  • One-step High Performance quantitative detection – no need to perform separate cDNA synthesis and qPCR
  • Results aligned with control standards included with the kit.
  • Low contamination risk – no post-PCR product handling
  • Fast – results in under 80 minutes after PCR start
  • Multiplexed – Control and target genes assessed in the same reaction
  • Includes all the reagent required to perform RNA to Result analysis no additional reagents needed. (Including Mastermix)

Kit Contents

  • Reagents for Nucleic acid extraction from clinical blood samples
  • Reagents for Real time PCR along with specific primer and probes
  • Inclusion of Exogenous Internal positive control reagents
  • Plastic wares for detection kit

Order Information

#ProductCat. No.Reaction size/kit
1Oncoscreen PML RARA bcr1 Quantitative PCR kitOPLQ2525
2Oncoscreen PML RARA bcr1 Quantitative PCR kitOPLQ5050
3Oncoscreen PML RARA bcr1 and bcr3 Quantitative PCR kitOPLSQ2525
4Oncoscreen PML RARA bcr1 and bcr3 Quantitative PCR kitOPLSQ5050
5Oncoscreen PML RARA bcr1 and bcr3 Quantitative PCR kitOPLSQ100100