MTHFR Genotyping Kit

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MTHFR GENOTYPING KIT

MTHFR stands for methylenetetrahydrofolate reductase. The MTHFR gene produces an enzyme responsible for a multi-step process that converts the amino acid homocysteine to another amino acid, methionine. The body uses methionine to make proteins and other important compounds. The disorders of folate metabolism caused by polymorphism of the MTHFR gene generally occurs through the mutation of C677T and A1298C p.Glu429Ala that reduces the functional ability of the MTHFR enzyme and causes an increase in levels of homocysteine in the blood as well as dysregulation of various important enzymes responsible to maintain the homeostasis in the body. MTHFR mutations have been linked to various adverse pregnancy outcomes: specifically, early fetal loss (most commonly defined as spontaneous abortion in the first or second trimester), late fetal loss (death in the third trimester), pre- ecclampsia, intrauterine growth retardation, placental abruption and neural tube defects.  Homozygotes for the c.665C>T variant have been significantly associated with elevated plasma homocysteine levels and with an increased risk for premature cardiovascular disease. These individuals may also show toxicity from methotrexate that affect folate metabolism used for treatment of AML, CML and arthritis.

Mylab Discovery Solutions’ Oncoscreen MTHFR genotyping Kit is designed to screen the polymorphism (C677T and A1298T) of MTHFR gene using a real time allele-specific multiplex PCR with human genomic DNA

Kit Features

  • Highly Sensitive allelic discrimination testing for MTHFR mutations C677T and A1298C mutation
  • Multiplex analysis with no need for optimization- Detects Wild and Mutant type in one go
  • Easy workflow with ready-to-use solutions and optimal quality control
  • Simple reaction setup and data interpretation
  • Includes all reagent required to perform Sample to Result analysis no additional reagents needed.
  • Results aligned with Wild and Mutant controls Included with the kit.
  • Low contamination risk – no post-PCR product handling
  • High-throughput – can screen up to 45 samples in a single run
  • Fast – results within 80 minutes after PCR start

Kit Contents

  • Reagents for nucleic acid extraction
  • Reagents for real time PCR
  • Quality Controls