PML RARA bcr1 and bcr3 Screening Kit

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PML RARA bcr1 and bcr3

Historically, Acute promyelocytic leukemia (APL or AML-M3) is one of the most lethal forms of acute myeloid leukemia with distinct clinical and histopathologic features.

Acute promyelocytic leukemia is characterized by a unique chromosomal translocation involving the retinoic acid receptor-alpha gene on chromosome 17 (RARA). In greater than 99% of cases of APL, retinoic acid receptor-alpha (RARA) gene on chromosome 17 is involved in a reciprocal translocation with the promyelocytic leukemia gene (PML) on chromosome 15, a translocation denoted as t(15;17)(q22;q12)

Depending on the location of breakpoints within the PML site, intron 6, exon 6 and intron 3, the respective PML-RARa transcript subtypes referred to as long (L or bcr1), variant (V or bcr2) and short (S or bcr3), may be formed representing 55%, 5% and 40% of the cases respectively. The presence of PML/RARA t(15;17) translocation is necessary for response to all-trans-retinoic acid and arsenic trioxide. Thus, the PML/RARA t(15;17) assay is useful for diagnosis and predicting treatment response. It is also helpful for monitoring therapeutic response and MRD and for detecting early relapse.

Kit Features

  • Easy workflow with ready-to-use solutions for “Direct to customer” clinical lab tests
  • Detection of the PML/RARA t(15;17) (q21;q22) translocation
  • a tool to detect for Acute promyelocytic leukemia (APL or AML-M3)
  • Real-time quantitative gene analysis of control gene and fusion gene transcript bcr1 (PMLex6/RAR ex3L-form (=BCR1) ) and fusion gene transcript bcr3 (PMLex6/RAR ex3S-form (=BCR3))
  • One-step High Performance quantitative detection – no need to perform separate cDNA synthesis and qPCR
  • Results aligned with control standards included with the kit.
  • Low contamination risk – no post-PCR product handling
  • Fast – results in under 80 minutes after PCR start
  • Multiplexed – Control and target genes assessed in the same reaction
  • Includes all the reagent required to perform RNA to Result analysis no additional reagents needed. (Including Mastermix)

Kit Contents

  • Reagents for Nucleic acid extraction from clinical blood samples
  • Reagents for Real time PCR along with specific primer and probes
  • Inclusion of Exogenous Internal positive control reagents
  • Plastic wares for detection kit

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Order Information

# Product Cat. No. Reaction size/kit
1 Oncoscreen PML RARA bcr1 Quantitative PCR kit OPLQ25 25
2 Oncoscreen PML RARA bcr1 Quantitative PCR kit OPLQ50 50
3 Oncoscreen PML RARA bcr1 and bcr3 Quantitative PCR kit OPLSQ25 25
4 Oncoscreen PML RARA bcr1 and bcr3 Quantitative PCR kit OPLSQ50 50
5 Oncoscreen PML RARA bcr1 and bcr3 Quantitative PCR kit OPLSQ100 100