HCV Genotyping Kit

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HCV Genotyping Kit

Hepatitis C virus (HCV) is a leading cause of chronic liver disease (CLD) that can progress to cirrhosis and hepatocellular carcinoma. HCV genotyping constitutes the basis for the clinical management of infected patients by providing a predictor of response to therapy and to decide on the length of time required for antiviral treatment.

HCV is classified into six major genotypes (1-6), many subtypes (a, b, c, etc.), and about 100 different strains (1,2,3, etc.) based on the genomic sequence heterogeneity. HCV genotype 3 and genotype 1 were found to be the predominant genotypes in the Indian sub-continent. Genotype 4 and genotype 6 appeared to show some geographic restriction. Genotype 1 and 4 are generally associated with a poor response to interferon alone, whereas genotypes 2 and 3 are associated with more favorable responses.

Mylab Discovery Solutions PathoDetect HCV genotyping kit utilizes real time RT PCR method for determining the genotype(s) of hepatitis C virus (HCV) in plasma from HCV-infected individuals. The test detects subtypes 1a and 1b of genotypes 1 using NS5B region and genotypes 2, 3, 4, 5, and 6 by targeting 5′ UTR region of HCV. The kit performance is verified with NIBSC 3rd HCV RNA Genotype Panel.

Kit Features

  • Direct to customer” clinical lab tests based on real time PCR
  • Easy workflow with ready-to-use solutions and optimal quality control
  • Specific detection of major genotypes
  • Includes all the reagents required to perform Sample to Result analysis no additional reagents needed.
  • Inclusion of endogenous internal control for checking PCR inhibitors
  • Low contamination risk – no post-PCR product handling
  • High-throughput and Fast – results in under 80 minutes after PCR start

Kit Contents

  • Reagents for viral RNA extraction
  • Reagents for real time RT PCR
  • Inclusion of internal control
  • Positive controls and negative control for test validity
  • Lab consumables include real time strips and caps

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Order Information

# Product Cat. No. Reaction size/kit
1 Pathodetect-HCV Genotyping PCR Kit PHCVGE25 25
2 Pathodetect-HCV Genotyping PCR Kit PHCVGE50 50
3 Pathodetect-HCV Genotyping PCR Kit PHCVGE100 100