BRAF Mutation Screening Kit

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BRAF Mutation Screening Kit

BRAF, a kinase-encoding gene from the RAS/RAF/MAPK pathway is recently being studied in relation to prognosis of colorectal cancer.

Ninety percent of the BRAF mutations consist V600E mutation (1799T>A) which causes the constitutive activation of the protein.The presence of BRAF mutation is significantly associated with right-sided colon cancers and is associated with decreased overall survival.

BRAF mutations were suggested to be a negative predictor of response to anti-EGFR therapy.

Kit Features

  • Highly Sensitive Mutation Screening kit
  • Multiplex analysis with no need for optimization- Detects Wild and Mutant type in one go
  • Easy workflow with ready-to-use solutions and optimal quality control
  • Inclusion of Reagents for endogenous control amplification as a quality check for extraction and PCR
  • Simple reaction setup and data interpretation
  • Includes all the reagent required to perform Sample to Result analysis no additional reagents needed.
  • Results aligned with Wild and Mutant controls included with the kit.
  • Low contamination risk – no post-PCR product handling
  • High-throughput and Fast – results in under 80 minutes after PCR start

SNP screening of BRAF V600E

BRAF SNP screening real-time PCR kit is a multiplex real-time PCR which uses a pair of primer with two fluorogenic probes based chemistry that uses the 5 ́ nuclease activity of Taq DNA polymerase.

BRAF mutation screening kit detects single nucleotide polymorphism (SNP) – 1799T>A responsible for mutation V600E and discriminates mutant allele from wild type in a single tube.The kit includes all the reagents from sample to SNP detection.

A combination of robust reagents for DNA extraction from clinical samples and validated primers, hydrolysis probes as well as hot start Taq polymerase for SNP detection ensures that kit leads to highly specific and sensitive results in short time.

Kit Contents

  • Reagents for isolating DNA from Clinical samples
  • Reagents required for BRAF mutations screening on RTPCR
  • Validated reaction controls

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Order Information

# Product Cat. No. Reaction size/kit
1 Oncoscreen BRAF Mutation Detection Kit (V600E) OBMD25 25
2 Oncoscreen BRAF Mutation Detection Kit (V600E) OBMD50 50